枸杞干果DNA不同提取方法的比较

Comparison of the Different Extraction Method for DNA from Dry Fruit of Lycium bararum L.

[目的]为枸杞DNA分子标记、种质资源研究及地道药材鉴别等提供技术支持。[方法]以枸杞干果为试材,分别采用常规CTAB法、高盐CTAB法、改进CTAB法、5×CTAB法、常规SDS法、改进SDS法等12种方法提取其基因组DNA,比较不同方法的提取效果。[结果]12种方法中,5×CTAB法、改进SDS法等4种方法的提取效果不理想,其余8种方法均能有效提取枸杞干果的基因组DNA,且8种方法所提DNA的质量和产量均无明显差异。[结论]所用12种DNA提取方法中,8种方法提取的枸杞干果基因组DNA能够满足RAPD试验分析的需要。

[ Objective ] The aim was to provide the technical support for the studies on DNA molecular marker and germplasm resources of Lycium bararum L. and the identification of genuine medicinal plant. [ Method] With dry fruit of L. bararum as the material, its genomie DNA was extracted by routine CTAB method, high-coneentration-sah CTAB method, improved CTAB method, 5 × CTAB method, routine SDS method and improved SDS method etc 12 kinds of method. The extraction effect of different method was compared. [ Result] In 12 kinds of extraction method, the extraction effects of 5 × CTAB5 method, improved SDS method etc 4 kinds of method were not ideal. The genomic DNA from dry fruit of L. bararum could be efficiently extracted by other 8 kinds of method, and the quality and yield of genomic DNA extracted by 8 kinds of method had no obvious differences. [ Conclusion ] In 12 kinds of extraction method for DNA, the genomie DNA from dry fruit of L. bararum extracted by 8 kinds of method could meet the requirement of RAPD analysis.