成年、新生豚鼠心脏缺血再灌注期间功能、代谢和形态改变

The Different Changes of Cardiac Functions, Metabolisms and Morphology in Adult and Newborn Guinea-pig Hearts Subjected to Ischemia Reperfusion

目的研究比较成年和新生豚鼠心脏缺血再灌注期间的功能、代谢、形态的不同改变，为心肌保护提供依据。方法建立离体心脏左心做功模型，以ＳｔＴｈｏｍａｓＨｏｓｐｉｔａｌｃａｒｄｉｏｐｌｅｇｉｃｓｏｌｕｔｉｏｎⅡ（ＳｔＴｈｏｍａｓⅡ号液）为心停搏液，测定成年豚鼠（Ａ组）和新生豚鼠（Ｂ组）心脏缺血前、后和再灌注时（或后）动力学，心肌腺苷酸和丙二醛的含量，并行电子显微镜观察。结果Ｂ组再灌注时（或后）的心功能恢复，心肌腺苷酸贮备和超微结构的改善均低于Ａ组（Ｐ＜０．０５），而丙二醛的含量高于Ａ组（Ｐ＜０．０５）。结论以ＳｔＴｈｏｍａｓⅡ号液为心停搏液时，新生豚鼠心肌在缺血再灌注期间受损程度重于成年豚鼠，氧自由基的产生在其中起重要作用。

Objective To study the different changes in adult and newborn guinea-pig hearts subjected to ischemia reperfusion, then to provide data for myocardial protection. Methods Isolated antegrade perfused heart model was established. All hearts were arrested by St Thomas Hospital cardioplegic solution No.2 cardiac functions, myocardial content of adenine nucleotide and malondialdehyde(MDA) and ultrastructure were investigated before, after ischemia and after reperfusion in adult (group A) and newborn (group B) guinea-pig hearts. Results The recovery percentage of cardiac functions and myocardial content of adenine nucleotide, and the improvement of myocardial ultrastructure after reperfusion in group B were inferior to those in group A. Myocardial content of MDA after reperfusion was significantly higher in group B than that in group A (P<0.05). Conclusion Because of production of oxyradicals, injury to the newborn guineapig hearts were severe than to adult ones subjected to ischemia reperfusion.