摘要
目的对125例不同类型的地中海贫血(地贫)杂合子进行临床血液学分析。方法用Coulter血球计数仪测定分析对象末梢血红细胞平均容量(MCV)和红细胞平均血红蛋白(MCH)。用醋纤薄膜电泳比色法及1分钟碱变性法分别测得HbA2和HbF含量。结果所有地贫杂合子的MCV、MCH明显小于正常值(P<0.001),符合小细胞低色素性贫血,同时发现不同的β地贫突变类型,其MCV也存在差异,β0突变杂合子MCV低于β+突变杂合子(P<0.03)。结论MCV和MCH的测定不仅可为地贫的临床诊断提供依据。
Objective To analyze the clinical hematological data of 125 heterozygotes for different kinds of thalassemias.Method Mean corpuscular volume (MCV) and Mean corpusular hemoglobin (MCH) of 125 heterozygotes for thalassemias were measured by an automatic Coulter analyzer.Hemoglobin A2 was determined spectrophotometrically after electrophoretic separation on cellulose acetate membrane.Hemoglobin F was determined by alkali denaturation test.Results Mean MCV and MCH in all the tested thalassemia heterozygotes were statistically significantly lower than those in normals (P<0.001).The result also showed that there was a significant difference of the MCV value between different βthalassemia mutations,i.e.,Mean MCV of β0thalassemia heterozygotes was statistically significantly lower than β+thalassemia heterozygotes (P<0.03).Conclusions The MCV and MCH are valuable parameters in clinical diagnosis for thalassemia heterozygotes.In addition,MCV provides information for the identification of thalassemia heterozygotes with mutational diversity.
出处
《现代诊断与治疗》
CAS
1998年第5期270-271,共2页
Modern Diagnosis and Treatment
关键词
贫血
地中海贫血
杂合子
血液学分析
Thalassemia Heterozygote Mean corpuscular volume Mean corpusular hemoglobin