图像分析技术在肾小球肾炎诊断中的意义

The Use of Image Analysis Technique in Diagnosis of Glomerulonephritis

目的研究Ⅲ、Ⅳ型胶原在ＭｓＰＧＮ中的变化情况，同时探讨图像分析仪在此病中的诊断意义。方法将临床确诊为系膜增生性肾小球肾炎（ＭｓＰＧＮ）的病例分为轻、中、重三组，从每组中随机抽取１０例作为实验组１、２、３，取３例正常肾组织作为对照组。然后对所选取的３３例分别进行光镜图像分析，间接免疫荧光检查。结果在Ｍａｓｏｎ染色的绿染部分分析结果中，中、重度ＭｓＰＧＮ与正常比较有显著性差异（Ｐ＜０．０５），ＰＡＳＭ黑染中，重度与正常比较有显著性差异（Ｐ＜０．０５）。间接免疫荧光见：对照组肾小球内未见Ⅲ型胶原，有少量Ⅳ型胶原分布于毛细血管基膜及系膜区，Ⅲ型胶原在中，重度ＭｓＰＧＮ中出现，Ⅳ型胶原随着病变进展明显增加。结论Ⅲ、Ⅳ型胶原的出现及增加在肾小球肾炎的病变过程中起着重要作用。图像分析技术对肾小球肾炎的诊断具有一定的临床价值。

Objective To study the change of types Ⅲ and Ⅳ collagen in MsPGN and the value of computer image analysis technique in the diagnosis of MsPGN.Method Clinically diagnosed as patients with MsPGN were divided into three groups:mild,moderate and advanced.Ten patients were taken in random from each group and been composed of test group 1,2,3 respectively and three cases of normal kidney tissue were selected as control.All the 33 specimens underwent imaging analysis and indirected immunofluoroscope.Results There were significant differences(P<0.05) between the moderate and the normal groups and between the advanced and the normal groups in Massons green staining,and significant difference(P<0.05) between the advanced and the normal groups in PASMs black staining.For indirect immunofluoroscope,type Ⅲ collagen was only presented in the moderate and advanced groups and type Ⅳ collagen was gradually increased with the progress of disease,while in normal group,the former was absent,the latter was a little.Conclusions The types Ⅲ and Ⅳ collagen play a role in pathogenesis and the progress of MsPGN.The diagnosis of glomerulonephritis by computer image analysis technique had a clinical value.