PTE患者整合素相关mRNA差异性表达的意义

The significance of the differential expression of surface receptors mRNA of integrins in pulmonary thromboembolism patients

目的检测肺血栓栓塞症(PTE)患者整合素相关mRNA表达水平,分析其差异表达的意义。方法随机取选20例PTE患者为PTE组;另选20例年龄、性别与之匹配的非PTE患者为对照组。提取外周血单个核细胞总RNA,应用人类全基因组表达谱芯片比较两组整合素相关mRNA表达的差异,并进行FQPCR验证芯片的可靠性。差异基因筛选标准为P<0.05。结果检测出白细胞相关整合素基因mRNA14条,PTE组mRNA表达部分高于对照组,主要为β1及β2组,差异具有统计学显著意义(P<0.05);血小板相关整合素基因mRNA11条,PTE组表达上调的整合素占分布于血小板的所有整合素的60%,主要为β1及β3组,有统计学显著意义(P<0.05);其他整合素相关基因mRNA11条中,亦有部分表达显著上调或下调(P<0.05),它们的主要配体均为纤维连接蛋白。结论PTE中分布于白细胞以及血小板的大部分整合素表达异常上调,纤维连接蛋白相关整合素表达亦有显著改变,PTE可能与两者介导的炎症反应、血小板聚集相关。

Objective To detect surface receptors mRNA expression of integrins in pulmonary thromboembolism,to analyze the significance of its differential expression. Methods Twenty patients with PTE were enrolled in our study and the other twenty patients without PTE were allocated as controls. Total RNA was abstracted from isolated mononuclear cells in peripheral blood samples of twenty patients and controls. Significant differential mRNA expres- sion of integrins receptors were analyzed by means of mRNA expression microarray. Results Fourteen pieces of mR- NA fragments associated with leucocyte were screened and part of the genes were up-regulated in PTE group compared with control group （P 〈 0. 05 ）. The up-regulated mRNA fragments associated with leucocyte were mainly groups β1 and β2. Eleven pieces of mRNA fragments associated with platelet were screened and 60% of the genes were up-regu- lated in PTE group compared with control group （ P 〈 0. 05 ）. The up-regulated mRNA fragments associated with platelet were mainly groups β1 and β3. Eleven pieces of other mRNA fragments were screened and part of the genes were up-regulated or down-regulated,of which ligands were mainly fibronectin. Conclusion A great part of integrins associated with leucocyte and platelet were up-regulated in VIE, and the integrins whose main ligand being fibronectin were also significandy different between PTE group and control group. Inflammation and platelet aggregation which was induced by them may be one important pathological mechanism of VIE.