沙眼衣原体热休克蛋白60抗体的测定

Detection of antibody to chlamydial heat shock protein 60

为了了解泌尿生殖道沙眼衣原体感染者对沙眼衣原体热休克蛋白６０（ＨＳＰ６０）的血清学反应，应用细胞培养、免疫印迹试验和微量免疫荧光试验（ＭＩＦ）对１７５例非淋菌性尿道炎（宫颈炎）（ＮＧＵ）、７３例盆腔炎（ＰＩＤ）、５２例不育症及３６例健康对照者进行检查。３组患者的沙眼衣原体感染率有显著差异（Ｐ＜０．０１）。１７．８％的ＰＩＤ、１３．５％的不育症和１．７％的ＮＧＵ患者出现ＨＳＰ６０抗体，而健康对照组则未检出此抗体（Ｐ＜０．０１）。ＭＩＦ高滴度者（≥１∶１６０）出现ＨＳＰ６０抗体的机率（３１．３％）明显高于低滴度者（＜１∶１６０）（２．８％）（Ｐ＜０．０１）。提示在有并发症的患者中ＨＳＰ６０抗体常见。检测ＨＳＰ６０抗体对预测沙眼衣原体感染慢性发展及并发症的发生有一定意义。

In order to study serologic reaction to chlamydial heat shock protein 60 (HSP60) in patients with urogenital C.trachomatis infection,three patient groups,non gonococcal urethritis(cervicitis) (NGU),pelvic inflammatory disease (PID),and infertility,and a healthy control group were evaluated,by application of cell culture for C. trachomatis, immunoblotting and micro immunofl uorescence assay (MIF).The difference of incidence of urogenital C. trachomatis infection was significant among three patient groups (P<0.01) .Of those patients,17.8% of PID sera, 13.5% of infertility sera, 1.7% of NGU sera,and none of healthy control sera were positive for chlamydial HSP60 (P<0.01). The response to HSP60 was more common in patients with MIF titers ≥1∶160 (15/48,31.3%)than those with MIF titers <1∶160 (8/288, 2.8%) (P<0.01). The results suggests that immune response to HSP60 are frequently seen in those with C. trachomatis associated complications. Reaction with HSP60 could serve as a prognostic indicator for poor chronic outcome of chlamydial infections.