生殖器上皮HPV感染皮损中p53蛋白过度表达的检测

Over expression of p53 in genital epidermisof HPV infection

采用免疫组化方法对６例鲍温样丘疹病和９例尖锐湿疣生殖器上皮人类乳头瘤病毒（ＨＰＶ）感染皮损进行了ｐ５３蛋白、ｐ２１蛋白和增殖细胞核抗原（ＰＣＮＡ）表达的检测。结果ｐ５３阳性率为：鲍温样丘疹病（３／６）、尖锐湿疣（６／９），ｐ５３阳性细胞多位于基底层以及棘细胞层的中下层，同时发现受ＨＰＶ感染的空泡样变性细胞核中有ｐ５３蛋白和ＰＣＮＡ的过度表达，说明ｐ５３蛋白表达与ＨＰＶ感染及角朊细胞异常增生有关。

In this study,using immunohistochemical stainning,we detected the expression of p53 protein, p21 and PCNA in 15 cases of HPV infected genital epidermal tissues. The results showed that p53 positive staining was found in 3 out of 6 patients with Bowenoid papulosis and in 6 out of 9 patients with condyloma acuminatum. Themajority of p53 positive keratinocytes located in the basal layer and suprabasal layer,the positive staining of p53, p21 and PCNA was also detected in some vacuolated cells respectively. The above results suggest that HPV might induce p53 protein overexpression.