雄附散对抗博莱霉素诱导的大鼠肺间质纤维化的作用机制

Role of Xiongfusan in preventing bleomycin-induced pulmonary fibrosis

目的探讨雄附散对大鼠肺间质纤维化干预的机制。方法将70只SD大鼠随机分为正常组,假手术组,模型组,醋酸泼尼松组(5.6mg/kg),雄附散高、中、低剂量组(1.4g/kg、0.7g/kg、0.35g/kg)。各组大鼠于造模后第2天开始连续4周灌胃(ig),给予生理盐水(0.014L/kg)或相应药物(0.014L/kg),28d后取右中肺组织进行HE染色和Masson染色及MDA含量、SOD和GSH-PX活性测定。结果HE染色和Masson染色均提示雄附散高剂量组肺组织无明显形态学改变,肺组织中SOD和GSH-PX酶活性明显高于其他各组(P<0.01),MDA含量明显低于各药物干预组(P<0.01)。结论雄附散可能通过提高受损组织中抗氧化酶活性水平来对抗肺组织纤维化过程。

Objective To study the role of Xiongfusan in preventing bleomycin-induced pulmonary fibrosis. Methods Seventy SD rats were randomly divided into blank control group, sham-operation group, model group, prednisone treatment group（5.6mg/kg）, and 3 Xiongfusan treatment groups （1.4g/kg, 0.7g/kg and 0.35g/kg）. Rats received normal saline （0.014L/kg） and corresponding drugs （0.014L/kg） respectively from day 2 after gastric gavage of bleomycin, once a day for 4 weeks. MDA level and enzyme activity of SOD and GSH-PX were measured in tissues taken from the right and middle lung stained with HE and Masson stain 28 days after treatment. Results HE and Masson staining showed no obvious morphologic changes in lung tissues of the high dose Xiongfusan group. The enzyme activity of SOD and GSH-PX was significantly higher in the high dose Xiongfusan group than in the medium and low dose Xiongfusan treatment groups （P〈0.01）. The MDA level was obviously lower in Xiongfusan treatment groups than in other groups （P〈0.01）. Conclusion Xiongfusan can prevent bleomycin-induced pulmonary fibrosis by increasing the expression of antioxidant enzymes in injured lung tissues.