原发性肝癌组织线粒体DNA含量检测及意义

Detection and significance of mitochondrial DNA content in primary liver cancer

目的检测原发性肝癌及其癌旁组织中线粒体DNA含量,探讨线粒体DNA含量变化与肝癌发生的关系。方法荧光实时定量聚合酶链反应(real-time PCR)法扩增22例肝癌及相应癌旁组织中线粒体DNA编码基因细胞色素C氧化酶Ⅱ(COⅡ),以核编码基因磷酸甘油醛脱氢酶(GAPDH)作为内参照,比较两种组织中线粒体DNA含量的差异。结果肝癌组织中线粒体DNA相对含量为156.54±107.54,癌旁组织线粒体DNA相对含量为290.24±187.37,差异有统计学意义(P<0.01)。结论肝癌组织线粒体DNA的含量与肝癌发生有密切关系,有望成为一种新的肝癌标志物。

Objective To detect mitochondrial DNA content changes within hepatocellular carcinoma（HCC） and paracancer liver tissues. Methods Real-time polymerase chain reaction（PCR） was applied to detect the cytochrome C oxidase Ⅱ （CO Ⅱ ） of mitochondrial DNA in 22 cases of HCC and corresponding paracancer liver tissues, and glyceraldehyde-3-phosphate dehydrogenase （GAPDH） was served as a quantitative standard marker. Results Mitochondrial DNA content of HCC was 156.54±107.54,which was 290.24±187.37 for corresponding paracancer liver tissues（ P〈0.01）. Conclusion HCC carcinogenesis is closely correlated with mitochondrial DNA content,and it is hopeful as a new tumor marker.