人类腺病毒5型早期区域1A激活基因阻遏子rs3753921多态性与中国北方汉族人群冠心病发病的关联研究

Association of rs3753921 polymorphism of cellular repressor of E1A-stimulated genes and coronary artery disease in Han population of North China

目的探讨人类腺病毒5型早期区域1A激活基因阻遏子(cellular repressor of E1A-stimulated genes,CREG)基因rs3753921单核苷酸多态与中国北方汉族人群冠心病发病的相关关系。方法对经冠状动脉造影证实的338例冠心病患者和287例健康对照者,采用聚合酶链反应-重测序法检测CREG基因rs3753921单核苷酸多态位点在两组间的基因型和等位基因分布。结果CREG基因rs3753921单核苷酸多态3种基因型(TT型,CT型和CC型)在冠心病组分布频率分别为65.7%,30.2%和4.1%,在对照组分别为59.2%,35.5%和5.2%,两组间的基因型分布皆符合Hardy-Weinberg平衡定律,3种基因型在两组间的分布差异无统计学意义(P>0.05)。C等位基因在冠心病组和对照组间的分布频率分别为19.2%和23.0%,差异亦无统计学意义(P>0.05)。按性别及年龄分层进行亚组分析,CREG基因rs3753921单核苷酸多态的基因型和等位基因频率在冠心病组和对照组间差异无统计学意义。结论CREG基因rs3753921单核苷酸多态性与中国北方汉族人群冠心病发病可能无相关关系。

Objective To assess the possible association between cellular repressor of EiA-stimulated genes （CREG） and coronary artery disease（CAD） in the Han population of North China. Methods A case-control study was conducted in 338 patients with angiographically documented CAD and 287 healthy control subjects free from CAD who had normal coronary angiograms. The rs3753921 single nucleotide polymorphism was selected and genotyped. Results The genotype frequencies of TT,CT and CC in the CREG rs3753921 polymorphism were 65.7% ,30.2% and 4.1% in CAD patients,59.2% ,35.5% and 5.2% in the controls,respectively （ P 〉0.05）. The frequencies of CREG C allele in CAD cases and controls were 19.2% ,23.0% ,respectively（ P 〉0.05）. Subsequent stratified analysis by gender and age also showed no statistical significance in the genotype frequencies and allele frequencies between two groups. Conclusion The result suggests that rs3753921 polymorphism of the CREG gene might not be associated with CAD in the Han population of North China.