加味麻杏石甘汤防治鸡传染性支气管炎的试验研究

Experimental Study of Chinese Herbal Medicine Modified Maxingshigantang on Avian Infectious Bronchitis

将240羽15日龄雏鸡随机分为6组，每组40羽，除健康对照组外，其余5组均每羽滴鼻接种鸡传染性支气管炎病毒0．3mL，建立人工感染模型，于攻毒48h后分别以0．01％利巴韦林和0．6％、0．4％、0．2％加味麻杏石甘汤进行干预，统计各组死亡率及药物的有效率和治愈率，并采用ELISA方法检测18、21、24、27、30日龄时各组雏鸡血清中IBV抗体水平．结果表明，加味麻杏石甘汤可明显提高雏鸡IBV抗体水平，对IB的总有效率达90％～95％，治愈率为82．5‰～87．5％．

Two hundred and forty 15-day-old chickens were divided into six groups randomly.Except for blank control group,other groups were inoculated with infectious bronchitis virus at 0.3 mL.Birds in drug therapeutic groups were intervened with 0.6%,0.4%,0.2% dose of Maxingshigantang as well as 0.01 percent of Tylosin at 48h post infection respectively.To determine the serum antibody of each group ELISA method was used at days 18,21,24,27,30 and the death rate,total efficiency and cure rate were obtained.The results indicate that,the total efficiency of Maxingshigantang groups is up to 90%-95%,cure rate is 82%-88.5%,and it can apparently improve the level of serum antibody.