肝损伤过程中eNOS和IL-10的基因表达对肝再生调控机制的探讨

Expression of eNOS and IL-10 gene in signal transduction for liver regeneration

目的探讨内皮型一氧化氮合酶（ｅＮＯＳ）和白细胞介素１０（ＩＬ１０）在肝再生信息传导中的作用。方法切除７０％肝脏，制备肝再生模型，注射５０％ＣＣｌ４和脾切除引起肝损伤和机体免疫功能低下，４８小时后采用逆转录ＰＣＲ和点杂交方法检测ｅＮＯＳ和ＩＬ１０ｍＲＮＡ基因表达，免疫组化ＬＳＡＢ法检测ＩＬ１０蛋白在肝内的分布，流式细胞仪（ＦＣＭ）检测再生肝ＤＮＡ含量，采用统计学方法进行再生肝组织ＤＮＡ含量与ＩＬ１０和ｅＮＯＳ变化的相关分析，以此判断ＩＬ１０和ｅＮＯＳ的变化对肝再生的影响。结果ｅＮＯＳ的表达同肝细胞的再生呈正相关，而ＩＬ１０的表达与其呈负相关。结论ｅＮＯＳ和ＩＬ１０对肝再生具有明显的调控作用，无论在生理或在病理状态下都是如此，并且二者呈现一种完全相反的调控方式。

Objective To detect the effects of eNOS and IL-10 on liver regeneration under physiological and pathological condition. Method The rat model of liver regeneration was made by partial(70%) hepatectomy. Some rats were injected with CCl_4 and performed splenectomy.Hepatocytes DNA replication was tested by flow cytometry method (FCM) and the gene expression of eNOS in liver cells were analysed using RTPCR.The IL10 gene expression in liver cells was shown by Dot Blot hybridization method.The synthesis and distribution of IL10 protein in liver tissue were examined by immune histochemical and morphometric method. Result The expression of eNOS gene promoted liver regeneration while the expression of IL10 gene inhibited it. Conclusion eNOS and IL10 play an important immunomodulatory role in liver regeneration under physiological or pathological conditions. The effects of eNOS and IL10 on liver regeneration differ and they show an opposite modulatory effects on liver regeneration. Such effects are affected by spleen and general immune system.