人肝癌裸鼠移植模型癌瘤及癌周组织雄激素受体的动态变化

Dynamic change of androgenreceptor in tumor and surrouding liver tissue of nude mice model with transplanted human HCC

目的了解雄激素受体（ＡＲ）在肝癌（ＨＣＣ）进展过程中的动态变化规律。方法使用人ＨＣＣ裸小鼠原位移植模型，动态测定癌瘤及癌周组织的雄激素受体ＡＲ含量的变化。结果原位移植后４周９０％的裸鼠可见瘤结节，１０周见肿瘤坏死，１２周癌瘤大片坏死。测定原位移植瘤ＡＲ，无论是癌瘤或癌周组织，其总量均在肿瘤生长初期最高，分别为１０２３２±２１４２ｆｍｏｌ／ｍｇ蛋白和７２４５±１０１１ｆｍｏｌ／ｍｇ蛋白，然后随时间递减，到第１０周为４０９８±２１１１ｆｍｏｌ／ｍｇ蛋白、５３３９±７０１ｆｍｏｌ／ｍｇ蛋白，每２周之间差异均有显著的统计学意义。胞核和胞浆变化相同。结论雄激素在肿瘤的发生过程中由于ＡＲ的存在而起促进作用；在成瘤后雄激素的作用可能减弱，故在成瘤后再作抗雄激素治疗可能是无效的。

Objective To research into the law of androgenreceptor (AR) dynamic change in developing course of human HCC. Method Dynamic measurement of AR in tumor and peritumorous tissue was performed in nude mice model with orthotopic transplanted human HCC. Result Tumor nodules were present in 90% nude mice 4 weeks after transplantation,tumor necrosis was found in the 10th week, and remarkable necrosis occurred in the 12th week. The quantity of AR in tumor and peritumorous tissue was highest in the early stage of tumor (10232±2142 fmol/mg protein and 7245±1011 fmol/mg protein respectively), and then decreased progressively with time to the 10th week (4098±2111 fmol/mg protein and 5339±701 fmol/mg protein respectively). The difference in AR between each two weeks was significant. Conclusion Androgen promotes the development of tumor because of existence of AR, and its action may decline after establishment of tumor. Therefore,antiandrogen treatment may be ineffective after establishment of tumor.