心房颤动患者碱性成纤维细胞生长因子及受体mRNA表达

mRNA expression of basic fibroblast growth factor and receptors in human atria during atrial fibrillation

目的检测碱性成纤维细胞生长因子(bFGF)及其受体FGFR-1和HSPG在心房颤动(房颤)患者心房组织中的mRNA表达,探讨房颤患者心房纤维化的分子机制。方法75例风湿性心脏瓣膜病(风心病)接受换瓣手术者分为3组,其中窦性心律组34例,阵发性房颤组11例,持续性房颤组30例,于术中获取右心耳组织,采用半定量RT-PCR技术测定bFGF、FGFR-1和HSPG mRNA含量。结果与窦性心律组比较,bFGF、FG-FR-1和HSPG的mRNA在阵发性(P<0.05)和持续性房颤组(P<0.05)的表达均明显上调。同时,bFGF的mRNA水平与房颤持续时间(r=0.330,P=0.005)及左心房内径呈正相关(r=0.342,P=0.003)。结论心房组织中bFGF、FGFR-1和HSPG的mRNA表达上调可能是导致房颤患者心房纤维化的分子机制之一,与房颤的发生和维持有关。

Objective To investigate the significance and alteration of mRNA expression of basic fibroblast growth factor （bFGF） and its receptors includes FGFR-1 and HSPG related to atrial fibrosis in patients with atrial fibrillation （AF）. Methods Seventy-five patients with rheumatic heart disease who underwent heart valve replacement surgery were divided into three groups. Thirty-four patients were in sinus rhythm group, 11 patients were in paroxysmal AF group and 30 patients were in persistent AF group. Semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） technique was used in the mR NA level of bFGF, FGFR-1 and HSPG, which were taken from the right atrial tissue samples. Results Compared to sinus rhythm group, the mRNA of bFGF, FGFR-1 and HSPG increased significantly in the persistent AF group （P（0.05） and the paroxysmal AF group （P〈0.05）. The mRNA level of bFGF was positively correlated with AF duration （r= 0. 330, P 0. 005） and left atrial dimension （r=0.34Z, P=0. 003）. Conclusion The increased level of bFGF, FGFR-1 and HSPG mRNA expression in atrium could be one of the molecular mechanisms of atrial fibrosis during fibrillation, which correlates with the initiation and maintenance of AF.