免疫组化和PCR-SSCP检测p53基因异常的一致性探讨

Study of the concordance of immunohistochemistry and PCR SSCP detecting p53 gene abnormality

目的：探讨免疫组化检测ｐ５３蛋白表达和ＰＣＲ－ＳＳＣＰ检测ｐ５３基因突变的一致性。方法：单克隆抗体ＤＯ－７检测８５例非小细胞肺癌（ＮＳＣＬＣ）的ｐ５３蛋白表达，ＰＣＲ－ＳＳＣＰ检测其中３１例腺癌的ｐ５３基因突变。结果：８５例ＮＳＣＬＣ中ｐ５３蛋白表达阳性率为６８％（５８／８５），３１例腺癌中ｐ５３蛋白表达阳性率为６１％（１９／３１），１４例（４６％）出现ｐ５３基因５～８外显子突变，ｐ５３蛋白免疫组化和ＰＣＲ－ＳＳＣＰ检测ｐ５３基因突变无显著相关（χ２＝０．１，Ｐ＝０．７６），其一致率为５２％。结论：ｐ５３蛋白表达并不能很好地反映ｐ５３基因突变。

Purpose To study the concordance of immunohistochemistry and PCR SSCP(polymerase chain reaction single strand conformation polymorphism) screening for p53 gene abnormality, to assess whether p53 immunostaining perfectly reflects p53 gene mutation. Methods Monoclonal antibody DO 7 detected p53 protein expression in 85 cases, and PCR SSCP detected p53 gene mutation in 31 cases of adenocarcinoma. Results 58(68%) of 85 cases NSCLC had positive p53 staining, 19(61%) of 31 cases lung adenocarcinoma had positive p53 staining, and 14(46%) of 31 cases showed mutation in exon 5 to exon 8 of p53 gene. There was no significant correlation between immunohistochemistry and PCR SSCP detecting p53 gene mutation (χ 2=0 1, P= 0 76), and the concordance rate was 52%. Conclusion p53 immunostaining is imperfect reflection of the prevalence of p53 gene mutation.