肝细胞移植治疗大鼠急性肝衰竭的疗效观察

Study on effect of hepatocytes transplantation in rats with acute liver failure

目的观察肝细胞移植治疗大鼠急性肝衰竭的疗效，探讨原代肝细胞提取方法，观察体外培养的大鼠肝细胞生存情况。方法利用胶原酶消化法分离大鼠肝细胞，用D一氨基半乳糖（D—Galae—tosamineN，D—GAIN）制作大鼠急性肝功能衰竭模型，并以此模型为基础行大鼠同种异体肝细胞移植实验。结果分离的肝脏实质细胞（Hepatocyte）的存活率在87％-95％。平均每只大鼠肝脏可分离出7．8×10^7个肝脏实质细胞。肝细胞移植组大鼠7天的存活率为54．2％（13／24），对照组8．3％（2／24），移植组大鼠存活率与非移植组大鼠存活率之间具有显著性差异（P〈0．005）。结论经腹腔移植同种异体肝细胞可明显改善D—GalN诱导的急性肝衰竭大鼠的存活率，腹腔肝细胞移植是治疗急性肝衰竭安全有效的方法。

Objective To study the effect of hepatocyte transplantation（HCT） intraperitoneal in rats with acute liver failure（ ALF） which were induced by D - galactosamineN, and to discuss whether this is an effective way. Methods ALF animals were induced by D - GAIN. HCT was received 48h after intoxication. Group Ⅰ received 2.5 ×10^7 hepatocytes intraperitoneally. Group Ⅱ received 2.5 mL IsIS intraperitoneally. The survival rates, liver function,liver histology and biologic characteristics of transplanted cells were assessed. Survival rate of group Ⅰ was much higher than that of group Ⅱ （54. 2% vs 8.3% ,P 〈 0. 005） ,the liver function and liver histology were partially improved in group Ⅰ as compared with group Ⅱ. Conclusion Survival rate of ALF rats intoxicated with D - GalN could be improved, the liver function and liver histology could also be partially improved, via intraperitoneal HCT.