凝血酶对食管癌细胞EC109细胞迁移的影响

Effect of Thrombin on EC109 Cells Migration

目的:探讨凝血酶对食管癌细胞EC109细胞迁移的影响。方法:EC109分别接种于96孔培养板各孔,贴壁后划痕,分别加入不同浓度的凝血酶,作用时间12、24、48、72h。同时设置水蛭素组、凝血酶+水蛭素组及无药组。拍照并测量计算细胞迁移距离。结果:凝血酶组各浓度在不同作用时间的迁移距离与水蛭素组、凝血酶+水蛭素组和无药组比有统计学意义(P<0.05),水蛭素组、凝血酶+水蛭素组与无药组比无统计学意义,凝血酶组各浓度间比无统计学意义。结论:凝血酶可增强EC109细胞迁移能力。

Objective：To investigate the effect of thrombin on EC109 cells migration in esophageal carcinoma. Me-thods：EC109 cells were cultured in a 96-well culture plate. The cell monolayer was scraped in a straight line to create a ＂scratch＂ with a 10 μL pipet tip. The EC109 cells were cultured with thrombin,hirudin,thrombin＋hirudin in different concentrations. After 12,24,48,72 hours,the image of the scratch was acquired,then the distances of EC109 cells migration were measured and calculated. Results：Following 12,24,48,72 hours incubation with thrombin,the EC109 cells migration was obviously better than of groups（P〈0.01）. The EC109 cells migration among different concentrations of thrombin had no statistical significance. Conclusion：The thrombin may enhance EC109 cells ceumigration.