东莨菪亭与橙皮素联用对小鼠抗炎作用的定量分析

Quantitative Analysis of Anti-inflammatory Effect of Scopoletin and Hesperetin in Combination to Mice

目的:研究白花马鞍藤中主要成分东莨菪亭和橙皮素的相互作用。方法:用巴豆油致小鼠耳肿胀模型研究东莨菪亭与橙皮素的量效关系;用Loewe Additivity模型定量分析东莨菪亭和橙皮素联用对小鼠耳肿胀的抑制作用。结果:东莨菪亭与橙皮素能分别抑制巴豆油所致小鼠耳肿胀,并呈剂量-依赖关系;小剂量东莨菪亭+橙皮素合用指数<1,大剂量时>1。结论:东莨菪亭与橙皮素各单用时对炎症有抑制作用,合用时小剂量有协同作用,大剂量有拮抗作用。

Objective：To investigate the interaction of major constituents in Ipomoea Pescaprae（scopoletin and hesperetin）. Methods：Dose-effect relationship of scopoletin and hesperetin was studied with croton oil-induced mouse ear edema. Using the model of Loewe Additivity,the inhibition on mouse ear edema by scopoletin and hesperetin in combination was analyzed quantitatively. Results：Scopoletin and hesperetin inhibited croton oil-induced mouse ear edema in a dose-dependent manner respectively. The combination index was less than 1 in low dose and more than 1 in high dose in combination of scopoletin and hesperetin. Conclusion：Scopoletin and hesperetin can inhibit inflammation individually. Combination of scopoletin and hesperetin produces synergistic effect in low dose but antagonistic effect in high dose.