TT病毒核酸的检测

Detection of TT virus DNA

目的初步测定献血员、慢性乙型肝炎、慢性丙型肝炎及慢性非甲～戊型，非庚型肝炎患者中ＴＴ病毒的感染情况，分析不同ＴＴ病毒感染者血清中ＴＴ病毒开放读码框架２区部分基因序列。方法以套式聚合酶链反应测定ＴＴ病毒核酸，取献血员（ＴＸ２）、慢性乙型肝炎（ＴＸ３）、慢性丙型肝炎（ＴＸ４）及慢性非甲～戊型，非庚型肝炎患者（ＴＸ１）各１例ＴＴ病毒ＰＣＲ阳性产物，以双脱氧核苷酸链终止法测定核苷酸序列。结果检测２０例献血员、２９例慢性乙型肝炎、３１例慢性丙型肝炎及３２份慢性非甲～戊型，非庚型肝炎标本，ＴＴ病毒核酸阳性者分别占５．０％（１／２０）、６．９％（２／２９）、２９．０％（９／３１）和３４．４％（１１／３２）。与Ｎ２２克隆相比，ＴＸ１、ＴＸ２、ＴＸ３与ＴＸ４在核苷酸水平的同源性分别为９５．４１％、９８．４７％、９７．４５％和９７．９６％。结论本组献血员、慢性乙型肝炎、慢性丙型肝炎及慢性非甲～戊型，非庚型肝炎患者中均存在ＴＴ病毒感染者。从本组不同ＴＴ病毒感染者血清中分离出４株ＴＴ病毒序列。

Objectives To detect the infective rates of TT virus among Chinese donors, patients with chronic hepatitis B, chronic hepatitis C and hepatitis of unknown etiology and to sequence the partial genome of TT virus in the serum from Chinese.Methods Nestedpolymerase chain reaction was performed to detect TT virus with the primers derived from the open reading frame 2. The PCR products respectively from a donor (TX2), a chronic hepatitis B patient (TX3), a chronic hepatitis C patient (TX4) and a hepatitis patient with unknown etiology (TX1) were sequenced by the dideoxy chain terminating method. Results The positive rates of 5.00%, 6.90%, 29.03% and 34.38% were found among donor, patients with chronic hepatitis B, chronic hepatitis C and hepatitis of unknown etiology. Compared with N22 clone, the nucleic acid sequences of TX1, TX2, TX3 and TX4 were 95.41%,98.47%,97.45% and 97.96%.Conclusions Infection of TT virus was found among Chinese donors and, patients with chronic hepatitis B, chronic hepatitis C and hepatitis of unknown etiology. Four Chinese isolates of TT virus were obtained and the partial genes from the open reading frame 2 of TT virus were highly conserved.