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人脑源性神经营养因子基因的定点突变 被引量：3

Site directed mutagenesis of human brain derived neurotrophic factor gene
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摘要 应用寡核苷酸诱导的定点突变和ＰＣＲ技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。 Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.Mutated genes were confirmed by nucleotide sequence analysis.This lays the foundation of structure and function studies of brain derived neurotrophic factor.
作者 李军 舒斯云 包新民 戚正武
机构地区 第一军医大学生化教研室 珠江医院神经生物学研究室 上海生化所分子生物学国家重点实验室
出处 《第一军医大学学报》 CSCD 1998年第3期187-189,共3页 Journal of First Military Medical University
关键词 脑源性 神经营养因子 基因定点突变 brain derived neurotrophic factor site directed mutagenesis
分类号 Q593.2 [生物学—生物化学] Q78 [生物学—分子生物学]
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第一军医大学学报
1998年 第3期

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