中心组合设计法优化超临界CO_2提取大黄蒽醌工艺

Optimization of Extract Process for Anthraquinones from Radix et Rhizoma Rhei by SFE-CO_2 with Central Composite Design

目的采用中心组合设计法优化超临界CO2技术提取大黄蒽醌的工艺。方法以提取温度、提取压力、夹带剂流速为自变量,大黄蒽醌提取率、纯度和提取总量为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用效应面图和重叠等高线图选取较佳工艺,并进行验证分析。结果确定超临界CO2提取大黄蒽醌最优工艺为超临界提取温度39.7℃、提取压力42.9MPa、夹带剂流速3.14mL·min-1或提取温度50.3℃、提取压力42.9MPa、夹带剂流速2.15mL·min-1。结论中心组合设计法能够优选超临界CO2提取大黄蒽醌的工艺,方法简便,参数设置精度更高。

OBJECTIVE To optimize the anthraquinones extract process from Radix et Rhizoma Rhei by SFE-CO2 with the central composite design（CCD）. METHODS Independent variables were extraction temperature,pressure and entrainer flow rate. Dependent variables were extracting ratio,purity and totle amont of anthraquinones. A two-order polynomial equation was fitted to the data and response surface method of CCD was used to optimize the extracting process. The verification test was carried out. RESULTS The optimum conditions were 39.7 ℃,42.9 MPa and 3.14 mL·min-1 or 50.3 ℃,42.9 MPa and 2.15 mL·min-1. CONCLUSION The CCD can be used to optimize the anthraquinones extract process by SFE-CO2. The methods were simple and precise.