摘要
目的探讨逆转录病毒载体介导的人野生型p53基因转移对预防血管成形术后再狭窄的作用。方法构建野生型p53基因逆转录病毒载体,通过逆转录病毒载体介导,将野生型p53基因转导至大鼠球囊损伤的颈动脉再狭窄模型中,21d后观察其对新生内膜形成的影响,并检测p53蛋白表达水平。结果构建和包装了人野生型p53基因的重组逆转录病毒载体,并在体外细胞中表达;用逆转录病毒载体转导野生型p53基因至大鼠球囊损伤的颈动脉,免疫组化检测表明转导血管局部表达人野生型p53蛋白,且与对照组相比,损伤血管新生内膜/中层面积比降低了44%。
Objective: Vascular smooth muscle cell (VSMC)proliferation after injury is important in the pathogenesis of restenosis after balloon angioplasty. In current study,we observed the effects of human wild type p53 gene transduction by retroviral vector on preventing restenosis following angioplasty.Methods: We constructed recombinant retroviral vector expressing human wild type p53. It was transfected into the SD rat carotid artery after carotid balloon injury and the restenosis model was estabished. We measured neotima/media area ratio and detected human wild type p53 protein expression in the local artery at day 21 after gene transfer.Results: We constructed and packaged recombinant retroviral vector expressing human p53 gene.Human wild type p53 gene was transfected into local carotid artery after the rat carotid balloon injury. Human wild type p53 protein expression was detected in local artery by immunohistochemistry. Neotima/media area ratio was significantly reduced (44%,P<0.01) compared with the control group.Conclusion: The results suggest a cytostatic gene therapy approach for restenosis after angioplasty.
出处
《第一军医大学学报》
CSCD
1998年第3期190-195,共6页
Journal of First Military Medical University
基金
广东省自然科学基金
关键词
基因治疗
再狭窄
P53基因
血管成形术
手术后
wild type p53 gene
gene therapy
restenosis
vascular smooth muscle cells