RP-HPLC测定人血浆中阿苯达唑及代谢物的浓度

Determination of Albendazole and Metabolites in Human Plasma by HPLC

目的建立RP-HPLC测定血浆中阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜浓度的方法。方法在改进文献报道的基础上,采用C2柱固相萃取法处理血浆,建立了精确、灵敏、重现性和专一性好的高效液相-紫外检测法(HPLC-UV)。色谱条件:分析柱:Agilent Zorbax RX-C8(4.6mm×250mm,5μm);柱温:室温;流动相:乙腈-NaAc缓冲液(0.1mol·L-1,HAc调节pH5.0)=45∶55,流速0.9mL·min-1,检测波长291nm。结果阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜的血药浓度线性范围分别为10～600,10～1000,10～300μg·L-1;方法回收率分别为92.23%～103.81%,99.59%～100.21%,96.89%～106.84%。批内精密度分别为2.03%～3.12%,2.05%～3.80%,2.79%～4.14%;批间精密度分别为3.20%～4.33%,2.05%～3.48%,2.87%～3.87%。结论本法灵敏、准确,可用于阿苯达唑的人体药动学研究。

OBJECTIVE To establish a RP-HPLC method for the determination of albendazole （ABZ）,albendazole sulphoxide （ABZSO） and albendazole sulphone（ABZSO2） in human plasma. METHODS The drugs was extracted from plasma with C2 column by SPE method. The compounds were separated on an Agilent Zorbax RX-C8 column（4.6 mm×250 mm,5 μm）by Waters 2690 HPLC. The mobile phase consisted of acetonitrile-NaAc buffer （0.1 mol·L^-1adjusted to pH 5.0 with acetic acid）. The detection wavelength was 291 nm. The flow rate was 1.0 mL·min-1. RESULTS A good linearity was obtained over the range of 10～600 μg·L^-1for ABZ,10～1 000 μg·L^-1for ABZSO and 10～300 μg·L^-1for ABZSO2. The recoveries of method were from 92.23%～103.81% for ABZ、 99.59%～100.21% for ABZSO,96.89%～106.84% for ABZSO2. The precisions of within-run were 2.03%～3.12%,2.05%～3.80% and 2.79%～4.14%,respectively,and the precisions of between-run were 3.20%～4.33%,2.05%～3.48% and 2.87%～3.87%,respectively. CONCLUSION The method is sensitive,accurate and reliable,and can be used in clinical research of ABZ.