扇贝糖蛋白的化学组成与抗肿瘤活性的研究

STUDIES ON THE CHEMICAL COMPOSITION AND AN-TITUMOR ACTIVITY OF THE GLYCOPROTEIN FROM CHLAMYS (AZUMAPECTEN)FARRERI

栉孔扇贝边经热水煮提,DEAE—纤维素及Sephadex G-100柱层析分离纯化后得栉孔扇贝糖蛋白Ⅰ[glycoprotein of Chlamys(Azumapecten)farreri Ⅰ,GCFⅠ],GCFⅠ经酶解后得栉孔扇贝糖蛋白Ⅱ(GCFⅡ),分别对GCFⅠ、GCFⅡ 进行化学组成分析及抗肿瘤活性研究。结果表明:GCFⅠ和GCFⅡ是蛋白组成、糖含量不同的糖蛋白,对小鼠S_(180)肉瘤均有明显的抑制作用,抑瘤率分别为47.29％、46.97％。药理实验提示:扇贝糖蛋白的抗肿瘤活性主要与分子中糖链的结构有关。

Glycoprotein of Chlamys ( Azumapecten ) farreril ( GCFI) was prepared from the skirt of scallop Chlamys (Azumapecten)farreri by extraction with hot water,isolation and purification with column chromatography 'on DEAE-cellulose and Sephadex G-100 successively. GCFI was hydrolyzes with enzymes to obtain glycoprotein of Chlamys (Azumapecten)farreri IKGCFID-The chemical composition and antitumor activity of GCFI and GCFII were studied. The result showed that GCFI and GCFII were two glycoproteins in which the composition and content of protein and sugar were different. They could obviously inhibit Sarcoma 180 cells proliferation of mice with the inhibition rate of 47. 29% and 46. 97%,respectively. Pharmacological test indicated that antitumor activity of GCFI and GCFII were associated with the structure of sugar in the molecules.