血清中丙二醛修饰低密度脂蛋白自身抗体的测定及意义

ELISA DETERMATION OF SERUM AUTOANTIBODY AGAINST MALONDIALDEHYDE MODIFIED LOW DENSITY LIPOPROTEIN

目的了解血清中丙二醛修饰低密度脂蛋白（ＭＤＡ－ＬＤＬ）自身抗体与动脉粥样硬化之间的关系。方法建立了血清中ａｕｔｏａｎｔｉ－ＭＤＡ－ＬＤＬ酶联免疫测定方法，并比较中老年（４５～６５岁）健康人、冠心病、高血脂和高血压病人血清中的ａｕｔｏａｎｔｉ－ＭＤＡ－ＬＤＬ水平。结果冠心病和高甘油三酯血症及混合型高血脂病人血清中的ＭＤＡ－ＬＤＬ自身抗体量明显高于健康人。结论血清中ａｕｔｏａｎｔｉ－ＭＤＡ－ＬＤＬ水平可能与冠心病。

PURPOSE To invetigate the relationship between autoanti-MDA-LDL and atherosclerosis. METHODS ELISA method of serum autoanti-MDA-LDL was established, and the concentration of MDA-LDL autoantibody in the healthy group, the cornory heart disease, hyperlipidemicas and hypertension groups (45～65 years old) was measured and compared. RESULTS In comparsion with the healthy group, the concentration of MDA-LDL autoantibody of the cornory heart disease, hypertriglyceromic and mixed hyperlipidemic group markedly increased. CONCLUSIONS The level of serum MDA-LDL autoantibody may be related to the cornory heart disease and the hyperlipidemicas.