右旋糖酐40、70对红细胞与内皮细胞粘附的影响

Influence of Dextran 40 and Dextran 70 on the Adhesion of Erythrocytes to Endothelial Cells

采用流室显微观察系统，定量研究右旋糖酐４０（ＤＸ４０）和右旋糖酐７０（ＤＸ７０）对红细胞与内皮细胞动态粘附特性的影响。统计分析处理数据，得到反映切应力与细胞间动态粘附数关系的经验公式及粘附特征常数ａ，ｂ值。ａ值反映红细胞的初始粘附数，ｂ值反映随切应力增大，粘附数减小的速率。ＤＸ７０实验组的ａ值大于ＤＸ４０组的，而ｂ值的情况相反。故结果显示切应力越大，粘附的红细胞越少；ＤＸ４０使红细胞的粘附明显减少；ＤＸ７０使红细胞粘附显著增加。由此提示不同长度的细胞桥接分子对红细胞的粘附影响不同。

Adopting the flow chamber realtime microrecordation system,we studied the dynamic effects of dextran 40(DX40) and dextran 70(DX70) on the adhesion of erythrocytes to monolayer of cultured human umbilical vein endothelial cells (HUVEC).The results demonstrated that erytherocytes pretreated with DX40 adhered to HUVEC less than the erythrocytes of the control group did,but DX70 increased the number of erythrocytes adhering to HUVEC.The higher the concentration of DX70 was,the more the erythrocytes pretreated with DX70 adhered to HUVEC.When shear stress increased,the decrease rate of the number of erythrocytes (pretreated with DX70) adhering to HUVEC declined with the increase of DX70 concentration.These indicate that DX40 as an expander of blood plasma is better than DX70.