刺五加对糖尿病动物模型的降血糖影响初探

Exploring Influence of Ciwujia on Blood Glucose in Diabetic Animal Model

为了探讨长白山天然野生刺五加对实验性糖尿病小鼠血糖的影响,试验选用昆明种小鼠,禁食12h,腹腔注射四氧嘧啶,选择性破坏胰岛β细胞,造成小鼠实验性糖尿病模型。取40只糖尿病模型小鼠随机分为刺五加高、中、低剂量组和四氧嘧啶模型组,另取重量相近的正常10只昆明种小鼠作为空白对照组。刺五加高、中、低剂量组分别给予5.00,1.25,0.25g/kg的刺五加水煎液灌胃,四氧嘧啶模型组和空白对照组给予同体积的生理盐水。连续灌胃15d,测定其空腹血糖,观察模型小鼠血糖变化。结果表明,刺五加高、中剂量组的血糖值分别为(13.51±1.42)mmol/L、(15.39±0.79)mmol/L,与模型对照组(19.62±1.28)mmol/L相比,极显著降低血糖水平(P<0.01)。低剂量组(17.39±0.64)mmol/L与模型对照组相比,差异显著(P<0.05)。由此可见,长白山野生刺五加对实验性小鼠具有降血糖作用。

The aim was to study the influence of natural wild Ciwujia from Changbai Mountain on the blood glucose in diabetic animal model in the trial. 40 Kunming mice were selected and fasted for 12 h. The experimental model of mice in diabetes was established by administrating abdominal cavity of alloxan and selective destructing of islet β cells. The trial animals were divided into four groups,namely high-dose（HD）,medium-dose（MD）,Low-dose （LD） and alloxan model（AM）,10 normal Kunming mice with proximate body weight were used as the control group. HD,MD and LD respectively was given by intragastric administration at dosage of 5.00,1.25,0.25 g/kg of Ciwujia decoction,AM and control group was treated by saline with the same volume and way. After continuously intragastric administration for 15 days,the fasting blood glucose was determined to observe its changes regularity of the mice. Results the blood glucose contents in HD and MD respectively were （13.51 ± 1.42） mmol/L,（15.39 ± 0.79） mmol/L,they in blood glucose were significantly lower that of the control （19.62±1.28） mmol/L （P〈0.01）. Meanwhile,the blood glucose in LD was （17.39±0.64） mmol/L,compared with the control,there was markedly different （P 〈0.05）. In conclusion,Changbai Mountain wild Ciwujia have the hypoglycemic effect to the experimental mice.