2株酸梅汤饮料污染霉菌的分离与鉴定研究

Studies on the Isolation and Identification of Contaminating Molds from Plum Juice Drink

目的:对酸梅汤饮料中的污染霉菌进行分离与鉴定。方法:采用涂布分离法分离污染霉菌,经形态学观察和ITS区rDNA序列分析对菌株进行鉴定。结果:从产生絮状沉淀的酸梅汤饮料中分离到2株霉菌,经鉴定这两株污染菌分别为黄暗青霉(Penicillium citreonigrum)和光孢青霉(Penicillium glabrum)。结论:根据两种污染菌的常见生境,酸梅汤饮料中的污染菌可能来自生产果汁的水果原料。

Objective：To isolate and identify contaminating molds from plum juice drink.Method：Contaminating molds were isolated by spread-plate method,and identified by ITS sequence analysis and morphological observation,respectively.Results：Two molds were isolated from plum juice drink with flocculus precipitate,and were identified as Penicillium citreonigrum and Penicillium glabrum respectively.Conclusions：Based on the common habitats of the two molds,the contamination of the two strains may come from raw material of Plum juice.