云南瑞丽静脉药瘾人群HIV1感染者毒株env基因V3区序列测定研究

Sequence Analysis on HIV1 env V3 Rigion from HIV1 Infected Intravenous Drug Users in Ruili City,Yunnan

为了解云南瑞丽静脉药瘾人群HIV1感染者毒株的分子流行病学特征 ,运用套式聚合酶链反应 (nestedPCR)对来自云南静脉药瘾人群的 16株HIV1env基因V3区进行扩增 ,并对扩增片段进行DNA序列测定和分析。结果显示 ,与本地区共享序列相比 ,16株HIV1膜蛋白V3区氨基酸序列的株间变异为 0 %～ 2 3% ,平均为 7%。HIV1云南株膜蛋白V3区氨基酸共享序列与HIV1SF2株及美欧株共享序列同源性在 90 %以上 ,而与海地、日本及非洲等地的代表毒株同源性较低。结果表明 ,在进化上这16株HIV1毒株间有非常密切的关系。这一地区的流行毒株在这一时期以美欧株、HIV1SF2株及其衍生株为主。

To study the molecular epidemiologic features of HIV1 epidemic strains from intravenous drug users (IVDUs) in Yunnan, nested primers polymerase chain reaction was developed for the amplification of env gene V3 fragment of HIV1 strains. DNA sequencing and analysis were performed with these fragments. Results showed that the variation of HIV1 gp120 V3 amino acids sequences among 16 HIV1 strains was averaged 7% (0%-23%),when comparing their consensus sequence. The amino acids consensus sequences of gp120 V3 region showed that more than 90% of homology compared with that of HIV1 SF2 strain and HIV1 American-European strain. However,lower homology with that of HIV1 strains from Haiti, Japan and Africa was revealed. The results suggested that there was a close relation among these 16 HIV1 epidemic strains isolated in Yunnan Ruili in terms of virus evolution. The epidemic HIV1 strains in the area were predominately American-European, SF2 and their derivatives in this period.