摘要
目的探讨卡托普利对糖尿病大鼠肾脏醛糖还原酶基因表达的影响及其意义。方法建立糖尿病大鼠模型,分为卡托普利干预组和对照组,15周后处死大鼠,从肾脏皮质抽提mRNA,逆转录分别用Cy5和Cy3标记,成为两组cDNA探针。cDNA探针与基因芯片杂交,扫描仪扫描,用软件进行分析。结果与对照组相比,干预组醛糖还原酶基因表达下调。结论卡托普利可能通过下调醛糖还原酶的表达产生肾脏保护作用。
Objective To study the effect of captopril on the expression of aldose reductase gene in renal tissue from diabetic rats. Methods Diabetic rats were divided into captopril intervention group and control group. All diabetic rats were killed 15 weeks after intervention,and eDNA probes were prepared by labeling the captopril intervened tissue mRNA and the eontroled tissue mRNA with fluorescent Cy5 and Cy3 respectively through reverse transcription. The eDNA probes were hybridized against the gene chip and then the fluorescent signals were scanned. Results compared with the control group,the aldose reduetase gene was down-regulated in the captopril intervention group. Conclusion Captopril may provide renal protection by down-regulating the expression of aldose reductase.
出处
《中国现代医生》
2009年第36期18-19,共2页
China Modern Doctor
基金
福建省卫生厅青年科研课题(项目编号:2003-1-2)
