胶原蛋白Ⅳ和层粘蛋白在膀胱癌基底膜中的表达及意义

Basement membrane expressions of collagen type Ⅳ and laminin in bladder transitional cell carcinoma

为了明确胶原蛋白Ⅳ（ColⅣ）和层粘蛋白（LN）在膀胱癌基底膜中的表达与含量，采用免疫组织化学方法（SABC法）测定35例膀胱移行细胞癌组织基底膜结构糖蛋白──ColⅣ、LN含量和分布。结果浅表性膀胱移行细胞癌基底膜ColⅣ和LN含量高，且呈连续分布；浸润性膀胱移行细胞癌基底膜ColⅣ和LN含量低，呈不连续分布；病理级别低、分化程度高的膀胱癌基底膜ColⅣ和LN与病理级别高、分化程度低的膀胱癌比较，前者的含量高、分布连续。提示结构糖蛋白──ColⅣ和LN在浸润性膀胱移行细胞癌基底膜中缺失，而在浅表性膀胱移行细胞癌基底膜中含量多，分布连续；基底膜ColⅣ和LN的含量随着分化程度的下降、病理级别的上升而减少。

In order to know the expressions of collagen type Ⅳ (Col Ⅳ ) and laminin (LN) at basement membrane in patients with bladder transitional cell carcinoma (BTCC),the distribution of Col Ⅳ and LN at basement membrane were studied by immunohistochemical techniques (SABC method) in 35 specimens of patients with BTCC. The result showed that the deeper the tumor invasion or the poorer the tumor differentiation,the more the loss of Col Ⅳ and LN at basement membrane,and the distributions of Col Ⅳ,LN between superficial tumor and invasive tumor were significantly different, respectively. It was concluded that the displays of Col Ⅳ and LN at basement membrane in patients with superficial BTCC were more than those in patients with invasive BTCC;the poorer the tumor differentiation,the more the loss of Col Ⅳ and LN at basement membrane.