免疫印迹检测抗眼肌抗体

Westerblotting for detection of antibodies against eye muscle antigens and its application

用含ＴｒｉｔｏｎＸ－１００及脱氧胆酸钠的ＴＤＯＣ缓冲液自牛眼肌提取了眼肌细胞膜抗原，建立了抗眼肌多肽抗体（ＥＭＡｂ）的免疫印迹检测法。结果表明６６．７％突眼性Ｇｒａｖｅｓ病患者（８／１２）和４２％Ｇｒａｖｅｓ病患者（３７／８８）血清均与６４ｋＤ、５５ｋＤ和４２ｋＤ的多肽反应；３９％ＳＬＥ患者（１５／３８）血清主要与４２ｋＤ及３５ｋＤ多肽反应。

The eye muscle membrane antigens were extracted from bovine orbital muscles by TDOC buffer containing detergent Triton X 100 and deoxycholate,and the Westernblotting for detection of antibodies against eye muscle antigen(EMAbs)was developed.The results indicated that EMAbs against 64 kD,55 kD and 42 kD polypeptides were present in both sera of 67%(8/12)of Graves patients with exophthalmos.EMAbs in the sera from of SLE patients wrer mainly reacted with 42 kD and 35 kD polypeptides.It was suggested that the heterogeneous EMAbs might associate with the variety of clinical manifestations and pathological mechanism and in autoimmune ophthalmopathy.