鼻咽癌组织端粒酶活性的研究

Study of telomerase activity in nasopharyngeal cacinoma

目的：探讨端粒酶活性与鼻咽癌的关系。方法：采用端粒重复序列扩增法（ＴＲＡＰ）测定６９例经病理确诊的鼻咽活检组织及两株鼻咽癌细胞株的端粒酶活性。结果：５１例鼻咽癌（ＮＰＣ）标本端粒酶活性阳性率为８８２％（４５／５１）；１８例鼻咽慢性炎症标本阳性率为１１１％（２／１８）；两株鼻咽癌细胞株ＣＮＥ２及ＳＵＮＥ均呈阳性。结论：本实验结果ＮＰＣ组织中的端粒酶活性阳性率为８８２％，ＣＮＥ２及ＳＵＮＥ的端粒酶活性均呈阳性。提示用ＴＲＡＰ测定端粒酶活性有可能作为鼻咽癌诊断的指标之一。

Purpose:To reveal the relationship between telomerase activity and nasopharyngeal carcinoma(NPC).Methods:69 cases of nasopharyngeal biopsy,diagnosed pathologically,were detected with TRAP(telomeric repeat amplification protocol)for their telomerase activity.Result:The positive rate of telomerase activity was 88.2% in NPC(45/51)and 11.1% in chronic nasopharyng itis tissue(2/18).Two cell lines of NPC,CNE 2 and SUNE,also had telomerase activity detected.Conclusions:Telomerase activity was highly expressed in NPC and the detection of telomerose activity by TRAP may have potential in the diagnosis of nasopharyngeal carcinoma.