Anti-μ诱导人类B淋巴细胞株MBC-1凋亡的研究

Anti-immunoglobulin M induce apoptosis in human B lymphoma line MBC 1

目的：研究抗人ＩｇＭ（ａｎｔｉμ）在体外对表面ＩｇＭ阳性的人类Ｂ淋巴瘤细胞株ＭＢＣ１细胞及Ｍｏｒａ细胞增殖活性的影响，并探讨其可能的作用机制。方法：应用细胞形态学、流式细胞仪分析及ＤＮＡ凝胶电泳等。结果：测得ａｎｔｉμ对ＭＢＣ１细胞增殖有抑制作用，并存在剂量和时间相关性，而对Ｍｏｒａ细胞则无此作用；并证实ＭＢＣ１细胞的死亡是通过细胞凋亡引起的，具有凋亡的分子水平和形态学特征。结论：ＭＢＣ１细胞可作为研究人类Ｂ细胞表面抗原受体交联所致凋亡的一个实验模式，并为探讨凋亡在个体发育时免疫耐受形成过程中所起的作用提供了良好的实验依据。

Purpose:To investigate the effect of anti-Ig antibody (anti μ) on proliferation of the human B lymphoma cell line MBC 1 and elucidate the possible mechanism. Method:Apoptosis was detected by the morphologic observation,flow cytometry assay and DNA gel electrophoresis. Results:Anti μ could inhibit the proliferation of MBC 1 cell and this inhibition was induced through apoptosis. Conclusions:The MBC 1 cell line could be used as an experimental model to study the apoptosis induced by ligation of the antigen receptors on B cells,thus suggesting that apoptosis may be involved in the establishment of B cell tolerance to self-antigen.