油菜蜂花粉酚类化合物的初步分离和抗氧化性研究

Purification and Antioxidant Activity of Phenol Compounds from Rape Pollen

采用甲醇提取油菜蜂花粉,提取物再经正丁醇萃取后,用聚酰胺层析得到各分离组分,并对各分离组分进行抗氧化性指标测定,并进行TLC、HPLC分析。结果表明:甲醇提取物酚类化合物含量为3.69%,正丁醇萃取物为6.32%,聚酰胺层析中80%和100%乙醇洗脱组分酚类化合物含量分别为15.92%和16.87%。同时,测定蜂花粉中各分离组分在浓度为1mg/ml时的还原力和DPPH·清除率,甲醇提取物还原力为26μgVC/mg,正丁醇萃取物为84μgVC/mg,80%和100%乙醇洗脱组分提高到154μgVC/mg和104μgVC/mg;甲醇提取物DPPH·清除率为83.45%,正丁醇萃取物为88.66%,聚酰胺层析中80%和100%乙醇洗脱组分DPPH·清除率分别为97.20%和94.34%。实验表明:蜂花粉经过甲醇提取、正丁醇萃取和聚酰胺层析后,80%乙醇和100%乙醇分离组分酚类化合物含量和抗氧化活性均显著性提高。

Rape pollen was sequentially extracted by methanol and n-butanol. Polyamide column chromatography was used to further purification of n-butanol extract. Elution fractions were analyzed using TLC and HPLC, and antioxidant activity of each extraction fractions was determined. Results indicated that contents of phenol compounds were 3.69% in methanol extract and 6.32% in n-butanol extract. After polyamide column purification, contents of phenol compounds were 15.92% and 16.87% in 80% and 100% ethanol elution fractions, respectively. The antioxidant activity was evaluated by scavenging rate of DPPH free radicals and reducing power. The reducing powers were 26, 84, 154μgVC/mg and 104μgVC/mg for methanol extract, n-butanol extract, 80% and 100% ethanol elution fractions, respectively. The scavenging rates of DPPH free radicals were 83.45%, 88.66%, 97.20% and 94.34% for ethanol extract, n-butanol extract, 80% and 100% ethanol elution fractions, respectively. The content of phenol compounds and antioxidant activity exhibited an obvious increase through purification. Therefore, phenol compounds in rape pollen were potential antioxidants.