黄伞菌丝体多糖的分离鉴定及其抗氧化性研究

Isolation, Identification and Anti-oxidant Activity of Polysaccharides from Pholiota adiposa Mycelium

黄伞菌丝体烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后DEAE-纤维素柱层析,分离得到两种多糖组分PAMPⅠ和PAMPⅡ。红外光谱分析表明PAMPⅠ和PAMPⅡ均为含有葡萄糖醛酸的β-D-吡喃葡聚糖。采用化学比色法测定黄伞菌丝体多糖在体外的总抗氧化能力、抑制活性超氧阴离子自由基的能力、清除羟自由基的能力及对小鼠肝组织中脂质过氧化产物丙二醛(MDA)含量的的影响。结果表明:一定浓度范围内不同纯度的黄伞菌丝体多糖均有总抗氧化能力,能清除O2·和·OH及抑制MDA的生成,且呈现一定剂量关系。表明黄伞菌丝体多糖在一定浓度范围内具有抗氧化作用。

Crude polysaccharides were obtained from Pholiota adiposa Mycelium through hot-water extraction and ethanol precipitation. Crude polysaccharides after removing protein through Sevage method were purified using DEAE-cellulose column chromatography to obtain two polysaccharide ingredients, designated as PAMP-I and PAMP-II. Glucuronic acid β-D-pyran-glucan was identified in both purified polysacchride components through IR spectrum analysis. Total anti-oxidant capability, scavenging capability to superoxide anion and hydroxyl free radicals, and effect of polysaccharides from Pholiota adiposa Mycelium on MDA content were evaluated using colorimetry. Results indicated that polysaccharides from Pholiota adiposa Mycelium had anti-oxidant capability such as scavenging free radicals and suppressing MDA generation in a dose-dependent mode.