固定化碱性蛋白酶制备麦胚降血压肽研究

Antihypertensive Peptides Preparation from Wheat Germ Protein with Immobilized Alkaline Protease

采用中空壳聚糖作为载体,通过双功能试剂戊二醛作偶联剂,将碱性蛋白酶连接到中空壳聚糖,制备固定化碱性蛋白酶。研究固定化碱性蛋白酶的部分性质及水解麦胚蛋白质条件。实验结果显示,1.5%戊二醛溶液活化的壳聚糖偶联1mg碱性蛋白酶,酶催化相对活性可达90%;固定化酶最适pH值为9.5,最适反应温度约65℃,催化水解麦胚蛋白质的水解率达16.5%约需10min;固定化酶与麦胚蛋白质的比达到1:5时,一次性获取含降血压肽水解物质的得率为15.6%。

Wheat germ protein was hydrolyzed with immoblized alkaline protease in an effort to prepare peptides with antihypertensive effect. The alkaline proteases were immoblized through binding to hollow globular chitosan with glutaraldehyde. Then the immobilized alkaline protease were characterized and the hydrolysis condition of wheat germ protein with it were optimized. Results showed that after 1.0 mg of alkaline proteases were immobilized with 1.5% glutaraldehyde, 90% of the enzyme activity was remained, with the optimum temperature 65℃ and the optimum pH 9.5. The ratio of hydrolysis with the immobilized enzyme for 10 min was 16.5%, and antihypertensive peptides yield was 15.6% when the ratio of enzyme to wheat germ protein was 1：5.