摘要
本实验对单增李斯特菌鞭毛蛋白提取方法进行研究,分别在23℃和37℃的条件下对单增李斯特菌(血清型IVb)进行扩增培养,发现23℃下单增李斯特菌产鞭毛的能力更强。运用酸解法处理单增李斯特菌后,分别通过超速离心法和硫酸铵沉淀法对鞭毛蛋白进行纯化,SDS-PAGE电泳以及电镜的实验结果表明,超速离心较硫酸铵沉淀法简便、耗时短、纯度高、产量高,适用于单增李斯特菌鞭毛蛋白的提取。
In this study, the extraction of flagellin from Listeria monocytogenes was studied. Listeria monocytogenes IVb was cultured at 23 ℃ and 37 ℃, respectively. Stronger flagellin-producing capability was observed at 23 ℃ of culture condition. Flagellin was purified through ultra-centrifugation and ammonia sulfate precipitation after acid hydrolysis treatment of Listeria monocytogenes. Results from SDS-PAGE and electronic microscope revealed that ultra-centrifugation combined ammonia sulfate precipitation was an applicable method for flagellin extraction because this simple and time-saving method could provide flagellin with high purity and high yield.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第23期262-265,共4页
Food Science
关键词
单增李斯特菌
酸解法
鞭毛
超速离心
Listeria monocytogenes
acid hydrolysis
flagellin
ultra-centrifugation
