摘要
利用随机扩增多态性DNA(RAPD)标记对7个猴头菇之间的亲缘关系进行研究,获得了猴头菇不同菌株的DNA指纹图谱。结果显示:14个随机引物中有3个随机引物的扩增产物的DNA条带表现出明显的多态性。这3个引物共扩增出44条带,其中36条为多态性条带,多态性位点比率为81.81%。同时利用NTSYSpc2.1生物软件分析7个供试菌株之间的遗传相似性,并绘制系统进化树。
The phylogenetic relationship of 7 Hericium erinaceuss strains was studied using randomly amplified polymorphic DNA （RAPD） markers. The DNA fingerprints of different strains showed that there was genetic diversity among the tested strains. The results indicated that 3 out of 14 random primers were polymorphic. These 3 random primers amplified 44 bands in total, 36 bands of which showed polymorphism and the polymorphic rate was 81.81%. Furthermore, the NTSYSpc 2.1 biology software was used to analyze the genetic similarity of the tested strains and to construct the phylogenetic tree. This study can provide the molecular evidences for variety identification and phylogenetic analysis of the Hericium erinaceus.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第23期270-273,共4页
Food Science
基金
国家自然科学基金项目(30740017)
鲁东大学创新团队建设项目
关键词
猴头菇
RAPD
系统进化分析
Hericium erinaceus
RAPD
phylogenetic analysis
