核心链霉亲和素的原核表达

Expression of Core-Streptavidin Gene in Prokaryotic Host

以链霉菌(Streptomyces avidinii)的基因组为模板通过PCR扩增得到354bp的核心链霉亲和素基因Stv13,并将其与pET22b+、pGEX-4T-1连接构建大肠杆菌表达载体,与P43连接构建枯草芽孢杆菌分泌型表达载体。大肠杆菌表达的核心链霉亲和素Stv13大部分以包涵体的形式存在,而枯草芽孢杆菌则无核心链霉亲和素Stv13分泌表达。

Core-streptavidin gene Stv13 （354bp） has been cloned from Streptmyces avidinii using PCR amplification. Plasmids were constructed through inserting the Stv 13 gene into pET22b＋ and pGEX-4T-1 vectors for expression in E. coli. Another plasmid was also constructed through inserting the Stv13 gene into P43 vector for expression in B. subtilis. Most of core-streptavidin gene Stv13 was expressed in inclusion body as an insoluble form in E. coli expression system. In contrast, little core-streptavidin gene Stv13 was expressed in B. subtilis expression system.