摘要
对新疆圆柏叶内生菌进行分离,确定分离条件,分离纯化得到7株菌株,采用打孔法对这7株菌的发酵液进行抑菌实验。结果表明:其中两株菌的发酵液对枯草芽孢杆菌、金黄色葡萄球菌呈现较强的抑菌活性。通过形态学观察和革兰氏染色,确定1号菌株为革兰氏阳性球菌,2号菌株为革兰氏阴性球菌,均无荚膜,无芽孢,不产酸,不产气,淀粉水解为阴性。
Seven bacteria strains were isolated form leaves of Sabina vulgaris Ant, and antibacterial activity of their fermentation products were evaluated according to the size of their anitbacterial circles. Totally 2 endophytic bacterium exhibited antibacterial activity, which could inhibit the growth of Bacillus subtilis and Staphylococcus aureus. Colonial morphology observation and Gram staining of the 2 endophytic bacterium showed that one bacteria was coccus shaped, gram-positive, and the other was coccus shaped and gram-negative, both without capsule and spore, and without producing acid and amylase.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第23期294-296,共3页
Food Science
基金
国家自然科学基金项目(30560004)
新疆大学青年教师科研启动基金项目(QN040122)
关键词
新疆圆柏
内生菌
分离
抑菌活性
Sabina vulgaris Ant
endophytic bacteria
isolation
antibacterial activity
