枯草芽孢杆菌发酵产碱性蛋白酶的研究

Preparation of Alkaline Protease by Fermentation of Bacillus subtilis

对枯草芽孢杆菌发酵产碱性蛋白酶的培养基成分及培养条件进行优化。在单因素试验的基础上通过正交试验确定发酵培养基各成分最适宜配比。采用此优化培养基对发酵菌株的培养条件进行单因素试验。结果表明最佳的发酵培养基成分为:酵母浸粉2%、蔗糖1.0%、吐温-800.5%、硫酸镁0.02%;发酵条件为:接种量4%(V/V)、起始pH9,在150ml的三角瓶中,装液量为25ml,发酵36h。在最佳培养条件下,碱性蛋白酶粗酶活力可达1670U/ml。

Medium compositions and fermentation conditions for producing alkaline protease through the fermentation of Bacillus subtilis were optimized by orthogonal design based on single factor experiments. Results indicated that optimal medium compositions were 2% yeast powder, 1.0% sugar, 0.5% Tween 80, and 0.02% MgSO4. The optimal fermentation condition was 4% inoculation amount in 25 ml of medium filled in 150 ml Erlenmeyer flasks at the initial pH 9 for 36 h of fermentation. The activity of alkaline protease obtained at this optimal fermentation condition was 1670 U/ml.