植物乳杆菌Lactobacillus plantarum GUO安全性研究

Mutagenicity and Virulence Evaluation of Lactobacillus plantarum GUO

对实验室筛选得到一株具有降胆固醇作用的植物乳杆菌GUO(Lactobacillus plantarum GUO)进行致突变性试验和毒力试验,以测定该菌株的食用安全性。致突变性试验结果表明:在加大鼠肝微粒体酶S9和不添加的条件下,与自发回变菌落数的差异皆不显著,且与剂量效应无关,而阳性对照的回变菌落数均显著增加(P<0.05),说明Lactobacillus plantarum GUO无致突变作用;毒力试验结果表明:Lactobacillus plantarum GUO低、高浓度培养液与各自对照组相比,对小鼠体重增加的影响无统计学意义(P>0.05),且受试小鼠无毒力性反应或死亡。

Mutagenicity and virulence of Lactobacillus plantarum GUO, a cholesterol-reducing bacterium, were evaluated by conducting Ames test and animal test, respectively. There were no statistical significances of revertant colony number between Lactobacillus plantarum GUO treated groups and the control group, with or without S9 presence, and in a group between different Lactobacillus plantarum GUO dosages, while significance were found between control and positive control group in the Ames test （P 0.05）. This indicated that Lactobacillus plantarum GUO was probably of no mutagenicity. No tested SD rats had virulence reaction or died, and no statistically significant body weight changing was observed.