钾法提取斑点叉尾鮰内脏油的工艺研究

Extraction of Fish Oil from Viscera of Channel Catfish through Potassium-method

以斑点叉尾鮰内脏为原料,采用一种改进的钾法制备鱼油。钾法提取鱼油的最佳工艺条件为水解pH7.0、料液比1:1.5(g/ml)、水解温度65℃、水解时间35min、氯化钾用量4%和盐析时间10min,该条件下鱼油的提取率达到82.09%,鱼油中的饱和脂肪酸相对含量为20.59%,单不饱和脂肪酸和多不饱和脂肪酸分别为54.48%和15.18%。∑n-3/∑n-6的脂肪酸比值达到3.29,明显高于其他淡水鱼类。以上结果表明,斑点叉尾鮰内脏是生产鱼油的良好来源。

Fish oil is usually extracted from the wastes during fish processing. In this paper, fish oil extraction from viscera of channel catfish, Ictalurus punctatus, through a modified potassium hydroxide hydrolysis method, was investigated. The best extraction efficiency was obtained at the hydrolysis condition of pH 7.0, 1：1.5 of viscera-water ratio, 65 ℃ for hydrolysis temperature, 35 min for hydrolysis time, 4% potassium chloride, and 10 min for salting-out. The extraction rate of fish oil was up to 82.09% under this optimal condition. The relative content of saturated fatty acids accounted for 20.59% of total fatty acids. The relative content of monounsaturated fatty acids and polyunsaturated fatty acids were 54.48% and 15.18%, respectively. The ratio of n-3 to n-6 polyunsaturated fatty acids is 3.29, which was higher than that of other fresh water fish. Therefore, viscera of channel catfish provided a good resource for fish oil production.