鲢鱼氨基酸生产工艺优化研究

Preparation Processing of Amino Acids from Silver Carp

采用木瓜蛋白酶、酸性蛋白酶、碱性蛋白酶、鲢鱼酶进行单因素试验和多因素正交试验,筛选出最佳工艺条件。结果表明:在最佳提取条件下,鲢鱼酶法水解氨基酸百分含量达到27.3%、木瓜蛋白酶15.4%、酸性蛋白酶13.1%、碱性蛋白酶不足1%,其中,鱼酶法最佳工艺为酶用量5%、固液比1:6、酶解时间8h。对氨基酸工业生产具有重要参考价值。

Amino acids are important medical products and nutritional supplements. Many drugs and health products also use amino acids as raw materials. Carp is a good raw material resource for amino acids due to plentiful resource of freshwater carp in China and its high protein content. In order to resolve problems corresponding to low extraction rate using traditional acid hydrolysis, a novel design with combinatorial traditional and untraditional ideas was applied to amino acid production. Papaln, acidic protease, alkaline protease, and silver carp enzyme were explored to produce amino acids through single factor and orthogonal experiments. Results indicated that extraction rate for amino acids were 27.3% by silver carp enzyme, 15.4% by papain, 13.1% by acidic protease and less than 1% by alkaline protease. All of these investigations provided important reference values to industrial production of amino acids.