木蹄层孔菌多糖分离纯化及其结构初步鉴定

Isolation, Purification and Identification of Polysaccharides from Fomes fomentarius

采用乙醇沉淀法,对木蹄层孔菌的发酵液进行多糖提取,用离子交换和凝胶层析对木蹄菌多糖进行纯化,并对多糖的结构、组成进行分析。结果表明,木蹄菌多糖只含一种多糖,采用薄层色谱法分析,结果显示木蹄菌多糖由半乳糖聚合而成,对木蹄菌多糖的红外光谱分析,显示该多糖是吡喃糖,由α-糖苷键连接,含酯胺等基团。

The crude polysaccharides were prepared by ethanol precipitation method from the ferment liquid of fomes fomentarius. The polysaccharides were further purified with ion exchange chromatography and gel chromatography. Apart from this, the structure of polysaccharide was analyzed as well as its composition. The result showed that Fomes fomentarius polysaccharide displayed high homogeneity and was comprised of single polysaccharide, which was formed through the polymerization of galactose. The structure was analyzed by IR suggesting that the polysaccharide was in the form of pyranose containing α-glycosidic bond and C=O group.