西伯利亚铁线莲地上部分有效成分分析及总黄酮的测定

Analysis of Active Ingredients and Total Flavonoids in Clematis sibirica (L.) Mill

对西伯利亚铁线莲的地上部分采取不同的方法进行有效成分分析,即水提法、醇提法、石油醚提取法,结果表明,西伯利亚铁线莲中含有丰富的有效成分,采用乙醇回流浸提法对西伯利亚铁线莲的不同部位进行了总黄酮的提取和测定,结果总黄酮的含量顺序为叶>果实>茎。

The active ingredients from above-ground parts were prepared using aqueous extraction and solvent extraction including ethanol and petroleum ether. The performing condition for solvent extraction of flavonoids was optimized by an orthogonal experiment design and the factors influencing the yields of flavonoids were also investigated including extraction time, temperature, solvent concentration and liquid/solid ratio. The results suggested the abundance of active ingredients in aboveground part of Clematis sibirica （L.） Mill. Analyses on the contents of flavonoids in the extracts from various parts of Clematis sibirica （L.） Mill showed that all the parts were rich in flavonoids while the contents varied and the order decreased as follows： leaf 〉fruit〉 stalk.