柱前衍生-HPLC分析天山花楸多糖中的单糖组成

Use of Pre-column Derivatization HPLC for Determination of Monosaccharide Compositions and Contents in Sorbus tianschanica Ruper. Polysaccharide

目的:测定分析天山花楸中的单糖组成。方法:天山花楸多糖样品用2mol/L硫酸溶液水解成单糖,用1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化,采用反相高效液相色谱法于波长245nm处检测各单糖。结果:天山花楸多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖7种单糖组成。结论:此法简单、快速、重现性好,可用于天山花楸多糖中单糖组成分析。

Objective：To determine the monosaccharide compositions in polysaccharide from Sorbus tianschanica Ruper.. Methods：Sorbus tianschanica Ruper. polysaccharide samples were hydrolyzed with 2 mol/L sulfuric acid solution, and derivatized with 1-phenyl-3-methyl-5-pyrazolone （PMP）, and further separated by RP-HPLC using a equivalent elution process detected at 245 nm. Results： Polysaccharide of Sorbus tianschanica Ruper. was composed of mannose, rhamnose, galacturonic acid, glucuronide acid, glucose, galactose, arabinose. Conclusion： The method was simple, fast and reproducible, and can be used in determination of polysaccharide compositions of Sorbus tianschanica Ruper..