摘要
采用改良的CTAB法,植物基因组DNA小量制备试剂盒,SDS法和高盐低pH值法4种方法提取安徽市场上8种大豆种子的基因组DNA,首次用降落PCR(TD-PCR)检测转基因成分,并对阳性产物进行酶切验证。结果表明改良的CTAB法是一种简单、高效的大豆DNA提取方法,能够满足各种分子生物学分析;TD-PCR显著提高了PCR扩增的特异性和效率,可有效检测大豆转基因成分,具有良好的应用前景。
The DNA geneome were extracted from 8 species of soybeans sold in commercial markets of Anhui province by Improved CTAB method, high salt and low pH method, plant genomic DNA mini-prep kit and SDS method. Touchdown polymerase chain reaction （TD-PCR） was applied to detect the genetically modified components in soybeans for the first time. The positive amplification productions were also analyzed by endonuclease. The experiment results showed that the improved CTAB method was suitable for all kinds of molecular analysis due to its simplicity and high efficiency. The TD-PCR was able to significantly improve the specificity and efficiency of PCR products. It would provide a new method for detection of genetically modified components.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第24期355-358,共4页
Food Science
基金
农产品生物化工教育部重点实验室(合肥工业大学)开放基金项目(NKF2004004)
安徽省自然科学基金项目(070413143)
