摘要
采用高效液相色谱(HPLC)法测定绍兴花雕黄酒中5-羟甲基糠醛(5-HMF)的含量。通过比较不同溶剂的提取率与直接过滤进样的效果后,选定酒样直接过滤进样测定的方法,用反相C18色谱柱分离,以甲醇-水(7:93,V/V)为流动相,在284nm波长条件下进行检测。该方法快速、准确,在2.512~80.384mg/L范围内线性相关系数(R2)为0.9998,平均回收率为99.95%,相对标准偏差为1.21%,方法的最低检测质量浓度为0.02512mg/L。同时,测得新酿9个月的绍兴花雕酒和3年陈成品花雕酒中5-HMF含量分别为7.6924mg/L和53.5569mg/L。
A new method for the determination of 5-hydroxymethylfurfural by reversed-phase high performance liquid chromatography in rice wine was developed. The detections of different solvent extracts and filtrate of sample were compared and the latter seemed to present the better result. The chromatography analysis was carried out using a C18 column at 284 nm and the mobile phase comprised methanol and water (7: 93, V/V). Within the linear range of 2.512~80.384 mg/L, the method was fast and accurate with the average recoveries rate and relative standard deviation of 99.95% and 1.21%, respectively. The limit of determination was 0.02512 mg/L. The contents of 5-HMF in rice wine with different aging time by 9 months and 3 years were 7.6924 mg/L and 53.5569 mg/L, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第24期369-371,共3页
Food Science